File:Detection-of-Heteroplasmic-Mitochondrial-DNA-in-Single-Mitochondria-pone.0014359.s003.ogv

English: Time-edited video of the mitochondrion extraction process. A UV laser pulse lyses a single HL-60 cell open. An organelle diffuses away from the lysed cell. The microscope stage is adjusted so the IR optical tweezer laser, fixed in the middle of the screen, traps the organelle. The bright field light is blocked and the 488 nm fluorescence laser, overlapped with the tweezer laser, illuminates the organelle to verify it is a mitochondrion particle. The bright field light is turned back on and the femtopipette tip is positioned to capture the mitochondrion. After capture the fluorescence laser is turned back on and the femtopipette tip is moved in a controlled fashion to verify the mitochondrion is trapped inside the femtopipette tip.