File:Defined-Essential-8™-Medium-and-Vitronectin-Efficiently-Support-Scalable-Xeno-Free-Expansion-of-pone.0151264.s005.ogv
Defined-Essential-8™-Medium-and-Vitronectin-Efficiently-Support-Scalable-Xeno-Free-Expansion-of-pone.0151264.s005.ogv (Ogg Theora video file, length 20 s, 604 × 480 pixels, 141 kbps, file size: 345 KB)
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[edit]DescriptionDefined-Essential-8™-Medium-and-Vitronectin-Efficiently-Support-Scalable-Xeno-Free-Expansion-of-pone.0151264.s005.ogv |
English: Human iPSCs (Gibco hiPSC line) cultured in spinner flasks with E8 medium and VtnB retain their differentiation potential. Cardiomyocyte differentiation was performed using Life Technologies Cardiomyocytes Differentiation Kit by plating microcarriers with hiPS cells in low-attachment plates. Beating cell-VtnM aggregates in low-attachment plate were observed at day 10 of differentiation. |
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Source | S2 Video from Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J (2016). "Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems". PLOS ONE. DOI:10.1371/journal.pone.0151264. PMID 27149065. PMC: 4801338. | ||
Author | Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 09:51, 13 October 2016 | 20 s, 604 × 480 (345 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Human iPSCs (Gibco hiPSC line) cultured in spinner flasks with E8 medium and VtnB retain their differentiation potential. |
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Author | Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Cardiomyocyte differentiation was performed using Life Technologies Cardiomyocytes Differentiation Kit by plating microcarriers with hiPS cells in low-attachment plates. Beating cell-VtnM aggregates in low-attachment plate were observed at day 10 of differentiation. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-03-21 |