File:Crystal-Structure-of-an-Ammonia-Permeable-Aquaporin-pbio.1002411.s008.ogv

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Original file(Ogg multiplexed audio/video file, Theora/Vorbis, length 38 s, 1,440 × 1,080 pixels, 1.11 Mbps overall, file size: 5.02 MB)

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English: MD simulation demonstrates spontaneous ammonia permeation. AtTIP2;1 monomer is displayed in side view with LCP-His 131 of the selectivity filter as well as Asn 83 and Asn 197 in the NPA region shown as sticks. An ammonia molecule (sphere representation) enters the main pore from the cytosolic side of the protein. During permeation of the pore, ammonia reorients such that its free electron pair points towards the asparagines at the protein center. In the course of the video, which corresponds to 2.94 ns, the imidazole group of LCP-His 131 can be observed rotating by 180° and back again.
Date
Source S1 Movie from Kirscht A, Kaptan S, Bienert G, Chaumont F, Nissen P, de Groot B, Kjellbom P, Gourdon P, Johanson U (2016). "Crystal Structure of an Ammonia-Permeable Aquaporin". PLOS Biology. DOI:10.1371/journal.pbio.1002411. PMID 27028365. PMC: 4814140.
Author Kirscht A, Kaptan S, Bienert G, Chaumont F, Nissen P, de Groot B, Kjellbom P, Gourdon P, Johanson U
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current21:54, 18 October 201638 s, 1,440 × 1,080 (5.02 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Stereo (MP3) 128 kbps Completed 08:15, 1 November 2023 1.0 s

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