File:Contribution-of-CXCL12-secretion-to-invasion-of-breast-cancer-cells-bcr3108-S3.ogv
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Contribution-of-CXCL12-secretion-to-invasion-of-breast-cancer-cells-bcr3108-S3.ogv (Ogg Theora video file, length 3.0 s, 500 × 504 pixels, 471 kbps, file size: 173 KB)
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DescriptionContribution-of-CXCL12-secretion-to-invasion-of-breast-cancer-cells-bcr3108-S3.ogv |
English: Supplemental movies 1, 2 and 3. The Neu-YB (Additional file 3) mouse strain tumors had more motility than the Neu deletion mutant (activated receptor) (Neu-NDL) (Additional file 1) or Neu-YD (Additional file 2) mouse strains in the tumor microenvironment. Tumors were exposed by performing skin flap surgery, and the murine tumor tissue was imaged with an Olympus FluoView FV1000-MPE multiphoton laser scanning microscope. Cyan fluorescent protein-expressing tumors were excited at a wavelength of 880 nm using a 25 × 1.05-numerical aperture water immersion lens objective with a 2.3× zoom. Time-lapse imaging was done over 30 minutes, collecting a 3D image stack through 100 μm every 2 minutes in 5 μm steps in the z axis. Arrows indicate areas of cell motility. Scale bar = 25 μm. |
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Source | Boimel P, Smirnova T, Zhou Z, Wyckoff J, Park H, Coniglio S, Qian B, Stanley E, Cox D, Pollard J, Muller W, Condeelis J, Segall J (2012). "Contribution of CXCL12 secretion to invasion of breast cancer cells". Breast Cancer Research. DOI:10.1186/bcr3108. PMID 22314082. PMC: 3496141. | ||
Author | Boimel P, Smirnova T, Zhou Z, Wyckoff J, Park H, Coniglio S, Qian B, Stanley E, Cox D, Pollard J, Muller W, Condeelis J, Segall J | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:54, 28 November 2012 | 3.0 s, 500 × 504 (173 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional files 3 |
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Author | Boimel P, Smirnova T, Zhou Z, Wyckoff J, Park H, Coniglio S, Qian B, Stanley E, Cox D, Pollard J, Muller W, Condeelis J, Segall J |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |