File:Comparative-Live-Cell-Imaging-Analyses-of-SPA-2-BUD-6-and-BNI-1-in-Neurospora-crassa-Reveal-Novel-pone.0030372.s013.ogv
Comparative-Live-Cell-Imaging-Analyses-of-SPA-2-BUD-6-and-BNI-1-in-Neurospora-crassa-Reveal-Novel-pone.0030372.s013.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 2.0 s, 462 × 462 pixels, 161 kbps overall, file size: 40 KB)
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[edit]DescriptionComparative-Live-Cell-Imaging-Analyses-of-SPA-2-BUD-6-and-BNI-1-in-Neurospora-crassa-Reveal-Novel-pone.0030372.s013.ogv |
English: A group of germlings demonstrating dynamics of BN-1-GFP recruitment during different stages of cell fusion. Taken together, this sequence demonstrates that BNI-1 is present at homing CAT tips, focuses at the attachment site, forms an opening ring of fluorescence during fusion pore formation, and finally disappears from the fusion site. Note, the cell in the middle becomes lifted upwards due to the torque forces transmitted upon cell-cell attachment, and thus disappears from the focal plane. This “spore-torque” is commonly observed when imaging germling fusion of N. crassa in liquid medium. |
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Source | Movie S10 from Lichius A, Yáñez-Gutiérrez M, Read N, Castro-Longoria E (2012). "Comparative Live-Cell Imaging Analyses of SPA-2, BUD-6 and BNI-1 in Neurospora crassa Reveal Novel Features of the Filamentous Fungal Polarisome". PLOS ONE. DOI:10.1371/journal.pone.0030372. PMID 22291944. PMC: 3265482. | ||
Author | Lichius A, Yáñez-Gutiérrez M, Read N, Castro-Longoria E | ||
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current | 23:15, 17 November 2012 | 2.0 s, 462 × 462 (40 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | A group of germlings demonstrating dynamics of BN-1-GFP recruitment during different stages of cell fusion. Taken together, this sequence demonstrates that BNI-1 is present at homing CAT tips, focuses at the attachment site, forms an opening ring of fluorescence during fusion pore formation, and finally disappears from the fusion site. Note, the cell in the middle becomes lifted upwards due to the torque forces transmitted upon cell-cell attachment, and thus disappears from the focal plane. This ?spore-torque? is commonly observed when imaging germling fusion of N. crassa in liquid medium. |
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Date and time of digitizing | 2012 |
Language | English |
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2012
application/ogg
a6a3e17e45ac6672a66ef9b3197537e43dd6c514
41,044 byte
2.0331972789115644 second
462 pixel
462 pixel
- Molecular cell biology
- Lamellipodia
- Videos of cell types
- Cell fusion
- Cell polarity
- Videos of cell division
- Videos of cytoskeletal proteins
- Fungal proteins
- Fungal gene expression regulation
- Video microscopy
- Neurospora crassa
- Protein transport
- Regeneration (biology)
- Fungal spores
- Videos of tissue distribution
- Media from PLOS ONE