File:Chondrogenic-and-Gliogenic-Subpopulations-of-Neural-Crest-Play-Distinct-Roles-during-the-Assembly-pone.0024443.s007.ogv

English: Epibranchial ganglion assembly in the prdm1anl3 mutant. Timelapse of confocal projections showing a prdm1anl3 embryo expressing TgBAC(neuroD:EGFP)nl1 in the region of the otic vesicle beginning at 31 hpf. Images were acquired every 8.4 minutes over ∼15 hours. As in the wild type, cells forming the major vagal ganglion upregulate EGFP as assembly commences. However, by 46.26 hpf, only the facial and large vagal ganglia can be identifiied. The major vagal ganglion is elongated compared to wildtype cohorts and shows no signs of segmentation. In addition, a small group of cells (asterisk; 46.26 hpf) is visible ventral to the otic vesicle in the location occupied by the glossopharyngeal and nascent minor vagal ganglia in wild type. Abbreviations: F = facial ganglion; V = vagal ganglia. Scale bar = 50 µm.