File:Cell-Cycle–Dependent-Differentiation-Dynamics-Balances-Growth-and-Endocrine-Differentiation-in-the-pbio.1002111.s018.ogv
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[edit]DescriptionCell-Cycle–Dependent-Differentiation-Dynamics-Balances-Growth-and-Endocrine-Differentiation-in-the-pbio.1002111.s018.ogv |
English: 3-D reconstruction of whole-mount imaged Hnf1bCreER;mT/mG E14.5 dorsal pancreas after 24 h of in vivo lineage tracing at clonal density. Imaris software was used to perform a 3-D reconstruction of dorsal pancreas after masking of background signal in the surrounding mesenchyme. Staining for E-Cadherin (white) enables outlining of the pancreatic epithelium, while membrane GFP (green) represents labelled clones. After 360° spinning, a SOX9 symmetric two-cell clone is shown in high magnification with SOX9 in blue. Next, a NEUROG3 symmetric two-cell clone is displayed in high magnification, showing NEUROG3 in magenta. Frame rate: 15 fps. |
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Source | S5 Movie from Kim Y, Larsen H, Rué P, Lemaire L, Ferrer J, Grapin-Botton A (2015). "Cell Cycle–Dependent Differentiation Dynamics Balances Growth and Endocrine Differentiation in the Pancreas". PLOS Biology. DOI:10.1371/journal.pbio.1002111. PMID 25786211. PMC: 4364879. | ||
Author | Kim Y, Larsen H, Rué P, Lemaire L, Ferrer J, Grapin-Botton A | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:28, 24 March 2015 | 27 s, 1,280 × 720 (10.56 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | 3-D reconstruction of whole-mount imaged Hnf1bCreER;mT/mG E14.5 dorsal pancreas after 24 h of in vivo lineage tracing at clonal density. |
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Author | Kim Y, Larsen H, Rué P, Lemaire L, Ferrer J, Grapin-Botton A |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Imaris software was used to perform a 3-D reconstruction of dorsal pancreas after masking of background signal in the surrounding mesenchyme. Staining for E-Cadherin (white) enables outlining of the pancreatic epithelium, while membrane GFP (green) represents labelled clones. After 360° spinning, a SOX9 symmetric two-cell clone is shown in high magnification with SOX9 in blue. Next, a NEUROG3 symmetric two-cell clone is displayed in high magnification, showing NEUROG3 in magenta. Frame rate: 15 fps. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-03-18 |