File:Cell-Cycle–Dependent-Differentiation-Dynamics-Balances-Growth-and-Endocrine-Differentiation-in-the-pbio.1002111.s016.ogv

English: Time-lapse movie exhibiting symmetric (P/P) and asymmetric (P/N) pancreatic progenitor divisions. Two cell divisions are tracked in this movie (blue spots and red spots). One daughter of red spot (top; NEUROG3+ from immunostaining in Fig. 1G) was back-tracked from the last frame of the movie, and the other daughter was forward-tracked after the division was monitored. By referring to immunostaining at the end of the time-lapse imaging (Fig. 1), blue spotted daughters are symmetrically fated (P/P), as pancreatic progenitors (SOX9+), whereas red spotted daughters are asymmetrically fated (P/N), one as pancreatic progenitor (SOX9+) and the other as endocrine progenitor (NEUROG3+). Pdx1tTA/+;tetO-H2B-GFP signal is presented in white channel. Time indicates a time-lapse elapsed time in h:min:s. Frame rate: 6 fps.