File:Calcium-sensing-receptors-signal-constitutive-macropinocytosis-and-facilitate-the-uptake-of-NOD2-ncomms11284-s2.ogv

English: Supplementary Movie 1 Persistence of macropinosomes and limited mixing with late endosomal/lysosomal contents during the first 15 minutes after uptake 70 kDa dextran. hMDMs were pulsed with Alexa 647-labeled 10 kDa dextran (0.025 mg/mL) for 1 hour followed by a chase at 37°C to label the late endosomal/lysosomal compartment (cyan, upper panel). The hMDMs were then incubated with rhodamine-labeled 70 kDa dextran (0.025 mg/mL) for 1 min at 37°C, in the presence of M-CSF (200 ng/mL) to label newly formed macropinosomes (red, middle panel). The cells were then washed and imaged on a heated stage acquiring an image every 30 seconds for 15 min. The video is displayed at 7 frames per sec. Scale bar = 10 μm.