File:Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s006.ogv

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Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s006.ogv ‎(Ogg Theora video file, length 50 s, 508 × 69 pixels, 78 kbps, file size: 477 KB)

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English: HCA undergoes fast retrograde transport in non acidic organelles. Motor neurons cultures were incubated with fluorescent HCA (green) and Lysotracker (red) for 30 min at 37°C under depolarising conditions (60 mM KCl). Cells were then washed and imaged. HCA undergo fast retrograde transport (from left to right) in non acidic organelles as demonstrated by the almost total lack of colocalisation with Lysotracker. Cell body is out of view on the right. Frames were taken every 4 s and played at 5 frames/s. This movie is a representative example of experiments performed on two independent primary motor neuron cultures, replicated twice.
Date
Source Movie S3 from Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G (2012). "Botulinum Neurotoxins A and E Undergo Retrograde Axonal Transport in Primary Motor Neurons". PLOS Pathogens. DOI:10.1371/journal.ppat.1003087. PMID 23300443. PMC: 3531519.
Author Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G
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Date/TimeThumbnailDimensionsUserComment
current17:00, 21 January 201350 s, 508 × 69 (477 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 240P 19 kbps Completed 04:15, 20 August 2018 7.0 s
Streaming 240p (VP9) 19 kbps Completed 08:01, 16 December 2023 1.0 s
WebM 360P 29 kbps Completed 22:34, 29 November 2023 1.0 s
Streaming 144p (MJPEG) 75 kbps Completed 06:51, 1 November 2023 1.0 s

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