File:Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s005.ogv

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Original file ‎(Ogg Theora video file, length 15 s, 994 × 90 pixels, 22 kbps, file size: 40 KB)

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English: HCA shares fast retrogradely transported organelles with the neurotrophin receptor p75NTR. Motor neurons seeded in MFC were incubated for 30 min at 37°C with fluorescent HCA (red) and a fluorescently-labeled antibody against p75NTR (green) in resting conditions, then washed and imaged. Both HCA and the antibody have been added to the axonal side only of the MFC. The two channels are merged in the movie. Yellow structures indicate double positive organelles. Cell body is out of view on the right. Frames were taken every 3 s and played at 5 frames/s. This movie is a representative example of experiments performed on two independent primary motor neuron cultures.
Date
Source Movie S2 from Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G (2012). "Botulinum Neurotoxins A and E Undergo Retrograde Axonal Transport in Primary Motor Neurons". PLOS Pathogens. DOI:10.1371/journal.ppat.1003087. PMID 23300443. PMC: 3531519.
Author Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G
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Date/TimeThumbnailDimensionsUserComment
current17:00, 21 January 201315 s, 994 × 90 (40 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 8 kbps Completed 04:15, 20 August 2018 2.0 s
Streaming 480p (VP9) 8 kbps Completed 10:30, 12 January 2024 0.0 s
VP9 360P 4 kbps Completed 04:15, 20 August 2018 1.0 s
Streaming 360p (VP9) 4 kbps Completed 09:50, 17 January 2024 1.0 s
VP9 240P 3 kbps Completed 04:15, 20 August 2018 2.0 s
Streaming 240p (VP9) 3 kbps Completed 08:01, 16 December 2023 1.0 s
WebM 360P 29 kbps Completed 17:02, 21 January 2013 1.0 s
Streaming 144p (MJPEG) 28 kbps Completed 06:51, 1 November 2023 1.0 s

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