File:Assembly-of-the-Murine-Leukemia-Virus-Is-Directed-towards-Sites-of-Cell–Cell-Contact-pbio.1000163.s008.ogv
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Assembly-of-the-Murine-Leukemia-Virus-Is-Directed-towards-Sites-of-Cell–Cell-Contact-pbio.1000163.s008.ogv (Ogg Theora video file, length 10 s, 428 × 235 pixels, 1.06 Mbps, file size: 1.27 MB)
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[edit]DescriptionAssembly-of-the-Murine-Leukemia-Virus-Is-Directed-towards-Sites-of-Cell–Cell-Contact-pbio.1000163.s008.ogv |
English: Cell-to-cell transmission of virus particles towards target cells following completion of assembly. A HEK293 cell generating MLV labeled with Gag-YFP (red) and expressing dynamin2-CFP (green) in contact with a noninfected target XC cell expressing mCAT1-CFP (green) was monitored using spinning disc confocal microscopy. The cell-cell contact was imaged over a period of 1 h and 35 min. A z-stack of red and green images was taken every 60 s and merged into a single file to generate a QuickTime video. The video represents first the “red/green” sequence followed by the “red only” to facilitate the detection of de novo assembly events. At the end of each sequence, a single image is presented to display the merged image of all frames of the time-lapse video. The video is played at 10 frames/s. The six labeled particles correspond to particles A–F in Figure 4A. |
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Source | Video S2 from Jin J, Sherer N, Heidecker G, Derse D, Mothes W (2009). "Assembly of the Murine Leukemia Virus Is Directed towards Sites of Cell–Cell Contact". PLOS Biology. DOI:10.1371/journal.pbio.1000163. PMID 19636361. PMC: 2709449. | ||
Author | Jin J, Sherer N, Heidecker G, Derse D, Mothes W | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 15:39, 16 November 2012 | 10 s, 428 × 235 (1.27 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Jin J, Sherer N, Heidecker G, Derse D, Mothes W |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Cell-to-cell transmission of virus particles towards target cells following completion of assembly. A HEK293 cell generating MLV labeled with Gag-YFP (red) and expressing dynamin2-CFP (green) in contact with a noninfected target XC cell expressing mCAT1-CFP (green) was monitored using spinning disc confocal microscopy. The cell-cell contact was imaged over a period of 1 h and 35 min. A z-stack of red and green images was taken every 60 s and merged into a single file to generate a QuickTime video. The video represents first the ?red/green? sequence followed by the ?red only? to facilitate the detection of de novo assembly events. At the end of each sequence, a single image is presented to display the merged image of all frames of the time-lapse video. The video is played at 10 frames/s. The six labeled particles correspond to particles A?F in Figure 4A. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009-07 |