File:An-open-source-toolbox-for-automated-phenotyping-of-mice-in-behavioral-tasks-Movie1.ogv

English: Scoring of object interaction. Overview of spatial object recognition scoring module, also applicable for other “interaction” tasks. Typically, a video recording contains multiple enclosed boxes and each box may contain variable number of objects in a particular spatial configuration. Using an initialization GUI, users define the number of boxes and the number of objects per box for an experiment and interactively define regions of interest. Once several videos are initialized, scoring is done as a batch job. An example of real-time tracking of mice is illustrated. Top panel: The centroid and head of the animal are automatically detected in each frame and marked in green and red dots. A vector in the direction of the animal's gaze is marked in red (vector magnitude increased for visual clarity). Interaction is scored when the gaze vector crosses a user-defined region of interest (glass and metal objects). The boundary of interacting object becomes highlighted in red during the movie. Bottom panel: The cumulative time spent in the arena during each bout of interaction. Movie is sped-up x3. Once videos are scored, users can quickly scroll through a set of frames labeled as interacting and verify the accuracy of the algorithm or remove any false-positives if needed.