File:Actin--and-Dynamin-Dependent-Maturation-of-Bulk-Endocytosis-Restores-Neurotransmission-following-pone.0036913.s010.ogv
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Actin--and-Dynamin-Dependent-Maturation-of-Bulk-Endocytosis-Restores-Neurotransmission-following-pone.0036913.s010.ogv (Ogg Theora video file, length 50 s, 640 × 360 pixels, 1.23 Mbps, file size: 7.3 MB)
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DescriptionActin--and-Dynamin-Dependent-Maturation-of-Bulk-Endocytosis-Restores-Neurotransmission-following-pone.0036913.s010.ogv |
English: Dynamin inhibition results in formation of plasma membrane tubular structures. NMJ preparations were pre-treated with 30 µM dyngo-4a for 40 min followed by prelabeling with 10 µM FM1-43 for 5 min. The preparation was extensively washed in normal Ringer's solution prior to stimulation at 20 Hz for 10 min. Confocal Z-stack imaging revealed numerous FM1-43-positive tubular membrane structures. 3-dimensional modeling of the tubular structures was performed using Imaris imaging software. |
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Source | Movie S3 from Nguyen T, Maucort G, Sullivan R, Schenning M, Lavidis N, McCluskey A, Robinson P, Meunier F (2012). "Actin- and Dynamin-Dependent Maturation of Bulk Endocytosis Restores Neurotransmission following Synaptic Depletion". PLOS ONE. DOI:10.1371/journal.pone.0036913. PMID 22629340. PMC: 3358275. | ||
Author | Nguyen T, Maucort G, Sullivan R, Schenning M, Lavidis N, McCluskey A, Robinson P, Meunier F | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:17, 17 November 2012 | 50 s, 640 × 360 (7.3 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Nguyen T, Maucort G, Sullivan R, Schenning M, Lavidis N, McCluskey A, Robinson P, Meunier F |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |