File:Actin--and-Dynamin-Dependent-Maturation-of-Bulk-Endocytosis-Restores-Neurotransmission-following-pone.0036913.s008.ogv
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Actin--and-Dynamin-Dependent-Maturation-of-Bulk-Endocytosis-Restores-Neurotransmission-following-pone.0036913.s008.ogv (Ogg Theora video file, length 30 s, 768 × 576 pixels, 311 kbps, file size: 1.11 MB)
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DescriptionActin--and-Dynamin-Dependent-Maturation-of-Bulk-Endocytosis-Restores-Neurotransmission-following-pone.0036913.s008.ogv |
English: Time-lapse imaging of nerve terminal morphological changes. The NMJ preparation was pre-labeled with FM1-43 by electrical stimulation (2 Hz for 5 min), and extensively washed in normal Ringer's solution. Nerve terminals were then stimulated at 20 Hz for 10 min and visualized by time-lapse confocal imaging starting from the final 4 min of stimulation. The asterisk denotes the end of the stimulation, after which the nerve terminal undergoes various remodeling stages: the progressive increase in plasma membrane diameter (bulging), the rapid increase in intraterminal fluorescence following the collapsing in plasma membrane diameter, and the appearance of the ‘bulk endosomes’. |
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Source | Movie S1 from Nguyen T, Maucort G, Sullivan R, Schenning M, Lavidis N, McCluskey A, Robinson P, Meunier F (2012). "Actin- and Dynamin-Dependent Maturation of Bulk Endocytosis Restores Neurotransmission following Synaptic Depletion". PLOS ONE. DOI:10.1371/journal.pone.0036913. PMID 22629340. PMC: 3358275. | ||
Author | Nguyen T, Maucort G, Sullivan R, Schenning M, Lavidis N, McCluskey A, Robinson P, Meunier F | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:17, 17 November 2012 | 30 s, 768 × 576 (1.11 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Nguyen T, Maucort G, Sullivan R, Schenning M, Lavidis N, McCluskey A, Robinson P, Meunier F |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Time-lapse imaging of nerve terminal morphological changes. The NMJ preparation was pre-labeled with FM1-43 by electrical stimulation (2 Hz for 5 min), and extensively washed in normal Ringer's solution. Nerve terminals were then stimulated at 20 Hz for 10 min and visualized by time-lapse confocal imaging starting from the final 4 min of stimulation. The asterisk denotes the end of the stimulation, after which the nerve terminal undergoes various remodeling stages: the progressive increase in plasma membrane diameter (bulging), the rapid increase in intraterminal fluorescence following the collapsing in plasma membrane diameter, and the appearance of the ?bulk endosomes?. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |