File:A-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s4.ogv

English: Supplementary Movie 2 Images of MDA-MB-231 cancer cells following intracellular operation of the DNAzyme motor, monitored over 60 min. MDA-MB-231 cancer cells were incubated with the DNAzyme motor for 2 h. The cells were washed and then Mn2+ and the operating buffer were added. Immediately after the addition of Mn2+, fluorescence images of the cells were repeatedly acquired for 60 min at a rate of 2 frames per min. The observed fluorescence in the cells is a result of the intracellular operation of the DNAzyme motor initialed by the target microRNA (miR-10b) in the cells and activated by the cofactor Mn2+. An Olympus IX-81 fluorescence microscope coupled with a Yokagawa CSU × 1 spinning disk confocal scanhead was used. The LMM5 laser transmission setting was 20 and the laser excitation time was 495 ms for each frame of the fluorescence images.