File:A-method-to-investigate-radial-glia-cell-behavior-using-two-photon-time-lapse-microscopy-in-an-ex-Movie1.ogv

English: 3D high resolution two-photon imaging of GFP-expressing cells in a developing spinal cord slice. Two-photon z-stack at a single time point is shown with GFP-expressing radial glial cells 84 h after electroporation at E14. The 3D imaging area of the tissue block is approximately 500 × 500 × 150 μm with high spatial resolution, even along the z-axis, as is evident when the z-stack is divided by a clipping plane (yellow frame) showing clearly identifiable individual fibers throughout the tissue depth. In the opening frame dorsal is toward the top and the pial surface is toward the right of the frame. Single grid square is 50 × 50 μm.