File:A-gene-trap-transposon-eliminates-haematopoietic-expression-of-zebrafish-Gfi1aa-but-does-not-mmc7.ogv

English: Movie 6 : qmc551: GFP-positive haematopoietic cells seed perivascular niches in the caudal haematopoietic tissue at 2 dpf and patrol the head tissues at 6 dpf. This movie is related to Figure 3. (A) Timelapse confocal microscopy on qmc551;flk1: tdTom double transgenic embryos starting from 48 hpf showing 2.0 µm thick optical slices through the caudal haematopoietic tissue. Here, GFP+ haematopoietic cells undergo dynamic interactions with tdTom+ ECs. One of the cells is highlighted with a yellow circle. Please note that ECs in the ventral wall of the caudal artery co-express the two transgenes. Anterior is to the left, dorsal is up. The embryo shown was immobilized in 1% low melting point agarose. (B) A 6 dpf qmc551 transgenic embryo was anesthetized and placed in a tiny depression in agarose under a fluorescent Nikon SMZ1500 dissection microscope using a Nikon DS-5Mc/DS-U1 camera setup. From minute 5 on, pictures of the head region of the embryo (facing right) were taken manually every 3 min. Images were imported into Photoshop. In Photoshop, the pictures were moved and rotated to correct for the drifting movement of the embryo under the microscope. Furthermore, annotations were added. All pictures were then imported into iMovie to generate the final video. Individual frames are also shown in Figure 3O.