File:A-coral-on-a-chip-microfluidic-platform-enabling-live-imaging-microscopy-of-reef-building-corals-ncomms10860-s3.ogv

English: Supplementary Movie 2 Microscopic view of coral calcification. High magnification view of the skeletal growth of a settled S. pistillata micropropagate. Here, a Coral on a Chip device holding a 6 days old micropropagate was kept on the microscope stage for a period of 25 h under a constant flow of FASW. Temperature was set to 24oC for the entire period. Light flux was maintained at 500 µmol photons m-2 s-1 for the first 12 h, followed by a 13 h dark period. Skeletal growth was captured during the dark period. Frames were captured at 10 min intervals using differential interference contrast microscopy with a 40X long working distance objective (NA 0.6). Variability in background shading is due to lateral movement of the coral's tissue. Twitching dark circles are shades cast by zooxanthellae above the focal plane. Scale bar is provided in Fig. 3e.