File:A-conserved-leucine-occupies-the-empty-substrate-site-of-LeuT-in-the-Na+-free-return-state-ncomms11673-s3.ogv

English: Supplementary Movie 2 The movie depicts the full transport cycle of the NSS family going from the outward-facing return state (presented here) through all known states of the NSS cycle (see supplementary discussion and supplementary figure 6 for further details). The movie 'morphs' between available structures - LeuT outward-facing, Na+-free return state (this study), LeuT outward-open, Na+-bound1 (PDB entry code 3TT1), LeuT outward-oriented substrate-occluded (PDB entry code 2A65), MhsT inward-oriented and substrate-occluded (PDB entry code 4US3), and LeuT inward-open apoform (PDB entry code 3TT3). For compatibility in morphing procedures, a leucine and sodium-bound inward-oriented and occluded state was modeled for LeuT based on the MhsT structure as described in Methods. The structures were aligned on the scaffold domain (TMs 3-4, 8-9). TM5 is shown as a cyan helix, TM1 as red helix, Na+ ions at Na1 and Na2 sites are shown in green, leucine in orange.