File:A-Sensitized-Screen-for-Genes-Promoting-Invadopodia-Function-In-Vivo-CDC-42-and-Rab-GDI-1-Direct-pgen.1005786.s008.ogv

English: AC invadopodia dynamics after loss of cdc-42 . Ventral view time-lapse showing an AC of a cdc-42 RNAi treated animal prior to BM breach. Invadopodia are marked by F-actin (mCherry::moeABD; left) and with spots (magenta; right) that are overlaid on fluorescence. Isosurface renderings built in place of F-actin were used to determine a threshold for assigning the spots that mark AC invadopodia. Loss of cdc-42 reduced the number invadopodia and decreased the rate of invadopodia formation but did not affect invadopodia lifetimes. Frames were acquired for 24 minutes in one-minute intervals using a spinning disc confocal microscope (CSU-10; Yokogawa Electric Corporation). The frames are played at 10 frames per second. This video corresponds to Fig 1E and 1F and Table 2. Scale bar, 5 μm.