File:A-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s009.ogv

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English: Related to Fig 3B —BAG3 depletion impairs mitotic spindle dynamics. Video microscopy of a representative siBAG3-treated HeLa cells expressing RFP-a-tubulin and GFP-actin, which show characteristic mitotic spindle rocking upon depletion of BAG3 (siBAG3). Mitotic cells were imaged for a 1 h-period at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective; single plane images are displayed at 7 frames/sec.
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Source S3 Movie from Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J (2015). "A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis". PLOS Genetics. DOI:10.1371/journal.pgen.1005582. PMID 26496431. PMC: 4619738.
Author Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J
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current12:45, 8 December 20154.1 s, 660 × 660 (230 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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