File:A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv001.ogv

English: Movie of ARF6 Activation during Phagocytosis A phase contrast movie of an RAW264.7 macrophage engulfing an IgG-coated RBC is shown in the panel to the left. I A, I D, and I F images were used to calculate the time-lapse R M images (center panel), E D images (right-hand panel) and E A images (unpublished data). Activated ARF6 can be seen at a site of ruffling (upper left of the macrophage, near the center of the panels). Phagocytosis of an erythrocyte, initiated below the ruffle, induced the activation of ARF6 at the leading edge of the phagosome pseudopod during the extension phase. ARF6 was deactivated as the phagosome sealed. All videos seen here play at 6 frames/s. Frames were collected at 30-s intervals.