File:A-Novel-Chemotaxis-Assay-in-3-D-Collagen-Gels-by-Time-Lapse-Microscopy-pone.0052251.s001.ogv

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A-Novel-Chemotaxis-Assay-in-3-D-Collagen-Gels-by-Time-Lapse-Microscopy-pone.0052251.s001.ogv ‎(Ogg Theora video file, length 12 s, 336 × 238 pixels, 85 kbps, file size: 121 KB)

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English: Directional migration of freshly isolated human neutrophils in 3D collagen matrix towards Il-8. Images were taken at 10× magnification, and correspond to the Z projection of 5 layers reported at a reduced time and spatial resolution with about one frame every two minutes, in order to minimize the file dimension. The first 30 minutes, where each frame has a white overlay showing the progressive time and a dimension bar, are representative of the unstimulated condition where a limited fraction of cells move randomly. The IL-8 solution was added to the reservoir at t = 30 min. In the second part of the movie where the overlay is in black, cells are under the effect of an IL-8 concentration gradient. An increase in cell motility, that has been quantified in terms of average velocity and fraction of motile cells, and a preferential movement toward the source of the chemoattractant, i.e. towards the bottom part of the image, can be clearly noticed.
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Source Movie S1 from Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S (2012). "A Novel Chemotaxis Assay in 3-D Collagen Gels by Time-Lapse Microscopy". PLOS ONE. DOI:10.1371/journal.pone.0052251. PMID 23284956. PMC: 3526591.
Author Vasaturo A, Caserta S, Russo I, Preziosi V, Ciacci C, Guido S
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current13:00, 5 January 201312 s, 336 × 238 (121 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 40 kbps Completed 01:19, 10 April 2024 2.0 s
Streaming 240p (VP9) 41 kbps Completed 04:42, 9 December 2023 1.0 s
WebM 360P 43 kbps Completed 12:32, 16 November 2023 0.0 s
Streaming 144p (MJPEG) 139 kbps Completed 08:08, 29 October 2023 1.0 s

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