File:A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv

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A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv ‎(Ogg Theora video file, length 6.0 s, 512 × 512 pixels, 3.54 Mbps, file size: 2.53 MB)

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English: Morphological changes and movement of cells in the presence of MVs. Time-lapse observation of a monolayer of Ca9.22 cells was monitored by an optical microscope (Olympus) equipped with a CCD camera (Andor) in the presence of highly purified MVs isolated by the SB technique. The protein concentration of MVs used in the assay was 20 µg/ml. Images were taken at 30 sec/frame for 30 min immediately after addition of MVs suspended in Eagle’s balanced salt solution (EBSS). The constructed time-lapse images are shown at 100 msec/frame (300 times the original speed). The width of the image is 170 µm.
Date
Source Video S1 from Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H (2014). "A Novel Approach for Purification and Selective Capture of Membrane Vesicles of the Periodontopathic Bacterium, Porphyromonas gingivalis: Membrane Vesicles Bind to Magnetic Beads Coated with Epoxy Groups in a Noncovalent, Species-Specific Manner". PLOS ONE. DOI:10.1371/journal.pone.0095137. PMID 24830438. PMC: 4022494.
Author Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current21:03, 22 May 20146.0 s, 512 × 512 (2.53 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 1.24 Mbps Completed 20:39, 15 August 2018 8.0 s
Streaming 480p (VP9) 1.24 Mbps Completed 03:26, 17 December 2023 1.0 s
VP9 360P 558 kbps Completed 20:39, 15 August 2018 7.0 s
Streaming 360p (VP9) 559 kbps Completed 17:09, 17 December 2023 1.0 s
VP9 240P 266 kbps Completed 20:39, 15 August 2018 4.0 s
Streaming 240p (VP9) 266 kbps Completed 04:36, 9 December 2023 0.0 s
WebM 360P 516 kbps Completed 21:04, 22 May 2014 5.0 s
Streaming 144p (MJPEG) 680 kbps Completed 08:06, 29 October 2023 1.0 s

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