File:A-Highly-Conserved-Poc1-Protein-Characterized-in-Embryos-of-the-Hydrozoan-Clytia-hemisphaerica-pone.0013994.s005.ogv

English: Comparison of gastrulation in an uninjected embryo (left) and one developed from an egg injected with 2mM Poc1-MO (right) filmed in parallel. The sequence covers 19 hours with images acquired using DIC optics every 4 minutes. The embryos were filmed in agarose wells to retain them in the observation field, but continue to swim and rotate around the embryonic polarity axis by ciliary beating. Gastrulation proceeds by cell ingression from the oral pole (top) in both embryos, but is much delayed and more irregular in the Poc1-MO embryo. Note that some undivided cells are already present in the blastocoel of the Poc1-MO embryo prior to gastrulation, and further accumulate during the period of the film, likely contributing to the gastrulation difficulties.