File:A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s008.ogv

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A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s008.ogv ‎(Ogg Theora video file, length 30 s, 504 × 354 pixels, 147 kbps, file size: 537 KB)

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English: Live cell imaging of ricin-FITC in the presence of JNA6 and TGN. Real time imaging of A549 cells visualizing ricin-FITC in green pre-mixed with JNA6 prior to adding to cells and TGN in red labeled with a transient transfection as described in Material and Methods. The total fluorescence for the TGN marker is always overwhelming compared to ricin-FITC and therefore the absolute co-localization between ricin-FITC and the TGN is not apparent in the raw images prior to processing. Real time images were processed prior to quantification as described in Materials and Methods to determine co-localization values, which are reported in Fig 4B. Total length of the time lapse is 120 min.
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Source S2 Movie from Herrera C, Klokk T, Cole R, Sandvig K, Mantis N (2016). "A Bispecific Antibody Promotes Aggregation of Ricin Toxin on Cell Surfaces and Alters Dynamics of Toxin Internalization and Trafficking". PLOS ONE. DOI:10.1371/journal.pone.0156893. PMID 27300140. PMC: 4907443.
Author Herrera C, Klokk T, Cole R, Sandvig K, Mantis N
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Date/TimeThumbnailDimensionsUserComment
current02:17, 13 October 201630 s, 504 × 354 (537 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 27 kbps Completed 15:44, 14 February 2023 2.0 s
Streaming 240p (VP9) 27 kbps Completed 02:59, 9 December 2023 1.0 s
WebM 360P 56 kbps Completed 12:02, 16 November 2023 1.0 s
Streaming 144p (MJPEG) 44 kbps Completed 06:36, 29 October 2023 1.0 s

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