File:16. Биуретска рекција.ogg

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Original file ‎(Ogg multiplexed audio/video file, Theora/Vorbis, length 1 min 54 s, 1,920 × 1,080 pixels, 5.2 Mbps overall, file size: 70.88 MB)

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English: Biuret reaction:

The presence of proteins in mushrooms can be proven by a Biuret reaction. A button mushroom or champignon (Agaricus bisporus) is used as an object in the current experiment.

A large fruiting body is cut in half. The first reagent, sodium hydroxide with a concentration of 1 mol/dm3 is applied using a clean pipette. A thin layer should cover the entire surface of the cut side of the mushroom. The second solution is applied with another pipette, copper (II) sulfate with concentration of 1 mol/dm3, again over the entire surface. Due to the presence of proteins, the color changes, from light-blue to dark-blue and purple. This change in color is due to the formation of a copper-protein complex.

Planned and performed by Slavica Tofilovska and Katerina Rusevska, Department of Biology, FNSM, Ss. Cyril and Methodius University, Skopje, Macedonia.
Македонски: Биуретска реакција:

Присуството на протеини во печурките може да се докаже со биуретска реакција, за што во овој експеримент се употребува шампињонот Agaricus bisporus.

Се зема едно големо плодно тело и се сече на половина. Со чиста пипета се зема од првиот реагенс, натриум хидроксид со концентрација 1mol/dm3 и внимателно се нанесува тенок слој на целата површина на пресечениот дел од печурката. Потоа со друга пипета се нанесува од вториот раствор, бакар (II) сулфат со концентрација 1mol/dm3, по целата површина. Поради присуството на протеини бојата се менува, од светло сина во темно сина и виолетова. Оваа промена на бојата се должи на формирањето на бакар-протеински комплекс.

Осмислено и изведено од Славица Тофиловска и Катерина Русевска, Институт за биологија, ПМФ, УКИМ, Скопје.
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Source Own work
Author Deni Ingilizovski

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current23:32, 12 November 20211 min 54 s, 1,920 × 1,080 (70.88 MB)Dandarmkd (talk | contribs)Uploaded own work with UploadWizard

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VP9 1080P 1.4 Mbps Completed 23:38, 12 November 2021 6 min 20 s
Streaming 1080p (VP9) 1.39 Mbps Completed 19:13, 18 December 2023 2.0 s
VP9 720P 622 kbps Completed 23:36, 12 November 2021 3 min 32 s
Streaming 720p (VP9) 617 kbps Completed 19:21, 7 December 2023 2.0 s
VP9 480P 337 kbps Completed 23:36, 12 November 2021 2 min 38 s
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Streaming 240p (VP9) 142 kbps Completed 19:05, 21 November 2023 3 min 19 s
WebM 360P 491 kbps Completed 23:34, 12 November 2021 1 min 13 s
Streaming 144p (MJPEG) 813 kbps Completed 15:42, 27 October 2023 6.0 s
Stereo (Opus) 1 kbps Completed 09:28, 9 November 2023 1.0 s
Stereo (MP3) 128 kbps Completed 12:14, 27 October 2023 2.0 s

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