File:“cAMP-Sponge”-A-Buffer-for-Cyclic-Adenosine-3′-5′-Monophosphate-pone.0007649.s006.ogv

English: Vectorial transfer of cAMP between connected cells. NCM460 cells stably expressing the EpacH30 cAMP sensor were transiently transfected with cAMP sponge. Here the microscope field contained an isolated control cell (on the left) and a couplet of cells in which only one cell expressed the sponge construct (on the right). The image sequence shows a significant delay in the PGE2 response of the coupled sponge-expressing cell compared to the two controls, with an apparent transfer of cAMP from the control (top cell in couplet) to the cell expressing the cAMP sponge (bottom). Time-lapse images represent pseudo-color of EpacH30 480 nm/535 nm FRET emission ratio, one frame every 10 seconds, total experiment duration 5.5 minutes.