User:Raulbot/gallery

Wed Jun 20 22:34:49 EDT 2007

• A rapidly moving neutrophil can be seen taking up several conidia over an imaging time of 2 h with one frame every 30 s.
• Neutrophils are not able to take up C. albicans yeasts despite several contacts in liquid media, in contrast to A. fumigatus conidia. In addition, no dragging of yeasts is visible here.
• Like neutrophils, RAW macrophages as seen here are unable to take up yeasts despite contacts. Imaging time was 3 h with an interval of 30 s after each frame.
• Neutrophils are seen here interacting with C. albicans in collagen matrix. In contrast to A. fumigatus conidia, most of the PMNs carry Candida, and a phagocytosis event can be seen at 142.5 min.
• A few RAW macrophages can be seen here carrying Candida yeasts. A large yeast is taken up by a cell at 30 min and is pulled up towards the cell. The observation was made over a period of 2 h with one frame every 30 s.
• A neutrophil is seen here with a heavy load of A. fumigatus conidia, which are dragged along with the cell. The PMN seems to selectively pick up conidia despite several contacts with Candida yeasts. The video was made over 2 h with a time lapse of one frame every 30 s.
• A neutrophil can be seen here selectively taking up Candida yeasts despite several contacts with A. fumigatus conidia in a 3-D collagen matrix. Imaging time was 2 h with an interval of 30 s after every frame.
• A single DC can be seen here interacting with conidia in liquid media and taking up two of them. The observation was made over a period of 3 h with a time lapse of 30 s.
• Two highly active alveolar macrophages can be seen ingesting conidia. Time lapse is 30 s per frame over 2.5 h.
• An active J774 macrophage is seen taking up at least three conidia in a cooperative manner. The J774 cells were treated with 5 ng/ml interferon-γ one night before filming with conidia. The observation was made over a period of 2.5 h every 30 s.
• A PMN can be seen here touching a conidium and even displacing it, but eventually moving on without further interaction. Imaging time was 3 h with one frame every 30 s.
• A single DC can be seen here efficiently taking up at least four conidia in its vicinity.
• Several neutrophils can be seen dragging clusters of conidia without actually phagocytosing them. A majority of conidia are attached to PMNs at the end of the video leading to the formation of an aggregate. Imaging time was 3 h with a time lapse of one frame every 30 s.
• A well resolved dendritic cell drags a conidium through a distance of up to 9 μm. The conidium, however, is not phagocytosed by the cell. The observation was made over 3 h with one frame every 30 s.
• A 3-D reconstruction of a z stack showing a fixed preparation of a PMN carrying five conidia intracellularly and one on the surface. The actin cytoskeleton of the PMN is seen here in green, while the conidia are seen in red.