File:Whole-central-nervous-system-functional-imaging-in-larval-Drosophila-ncomms8924-s4.ogv

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Original file (Ogg Theora video file, length 56 s, 780 × 1,020 pixels, 5.03 Mbps, file size: 33.62 MB)

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English: Supplementary Movie 1 Whole-CNS functional imaging with one-photon multi-view lightsheet microscopy (ΔF/F overlay) Whole-CNS functional imaging at 5 Hz of a Drosophila 3rd instar larva expressing 57C10- GAL4,UAS-GCaMP6s, using high-speed simultaneous multi-view light-sheet microscopy. Panels show maximum-intensity projections of ΔF/F (color look-up-table) and CNS anatomy (grey) from dorsal (left), lateral (bottom right) and frontal views (top right). The CNS anatomy shown in grey represents GCaMP6s baseline fluorescence, which was gamma-corrected to reduce image contrast and instead highlight overall CNS morphology. Imaging was performed with one-photon excitation at 488 nm, maintaining a constant imaging speed of 370 frames per second (491 MB/s) over a period of one hour. The Movie shows several representative motor sequences (forward and backward waves) recorded during this one-hour observation period. Time is shown relative to the onset of imaging. The average GCaMP baseline intensity level in this recording is shown as a function of time in Supplementary Figure 8 (blue).
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Source Video file from Lemon W, Pulver S, Höckendorf B, McDole K, Branson K, Freeman J, Keller P (2015). "Whole-central nervous system functional imaging in larval Drosophila". Nature Communications. DOI:10.1038/ncomms8924. PMID 26263051. PMC: 4918770.
Author Lemon W, Pulver S, Höckendorf B, McDole K, Branson K, Freeman J, Keller P
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Date/TimeThumbnailDimensionsUserComment
current09:55, 31 October 201656 s, 780 × 1,020 (33.62 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 957 kbps Completed 02:17, 27 October 2018 2 min 20 s
VP9 480P 438 kbps Completed 02:16, 27 October 2018 1 min 36 s
VP9 360P 218 kbps Completed 02:15, 27 October 2018 1 min 4 s
VP9 240P 118 kbps Completed 02:15, 27 October 2018 40 s
WebM 360P 494 kbps Completed 09:56, 31 October 2016 56 s
QuickTime 144p (MJPEG) Not ready Unknown status

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