File:Visualization-of-Content-Release-from-Cell-Surface-Attached-Single-HIV-1-Particles-Carrying-an-pone.0148944.s001.ogv
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Visualization-of-Content-Release-from-Cell-Surface-Attached-Single-HIV-1-Particles-Carrying-an-pone.0148944.s001.ogv (Ogg Theora video file, length 15 s, 652 × 396 pixels, 11.45 Mbps, file size: 20.54 MB)
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[edit]DescriptionVisualization-of-Content-Release-from-Cell-Surface-Attached-Single-HIV-1-Particles-Carrying-an-pone.0148944.s001.ogv |
English: Extra-viral pH-sensor blinks as HIV-1 pseudoparticle shuttles between neutral and acidic cellular compartments. HXB2 pseudoparticles labeled with releasable content marker Gag-imCherry (red) and extra-viral pH sensor EcpH-ICAM (green) were pre-bound in the cold to CV1/CD4/CXCR4 cells and imaged for 1 hour at 37°C. Particle trafficking to mildly acidic cellular compartment completely quenches fluorescence from the EcpH-ICAM label. ~0.8% of particles exhibited dequenching of EcpH-ICAM following quenching, suggesting trafficking of the particle back to a neutral compartment or the cell surface. |
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Source | S1 Movie from Sood C, Marin M, Mason C, Melikyan G (2016). "Visualization of Content Release from Cell Surface-Attached Single HIV-1 Particles Carrying an Extra-Viral Fluorescent pH-Sensor". PLOS ONE. DOI:10.1371/journal.pone.0148944. PMID 26863211. PMC: 4749635. | ||
Author | Sood C, Marin M, Mason C, Melikyan G | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 06:39, 29 February 2016 | 15 s, 652 × 396 (20.54 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Extra-viral pH-sensor blinks as HIV-1 pseudoparticle shuttles between neutral and acidic cellular compartments. |
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Author | Sood C, Marin M, Mason C, Melikyan G |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | HXB2 pseudoparticles labeled with releasable content marker Gag-imCherry (red) and extra-viral pH sensor EcpH-ICAM (green) were pre-bound in the cold to CV1/CD4/CXCR4 cells and imaged for 1 hour at 37°C. Particle trafficking to mildly acidic cellular compartment completely quenches fluorescence from the EcpH-ICAM label. ~0.8% of particles exhibited dequenching of EcpH-ICAM following quenching, suggesting trafficking of the particle back to a neutral compartment or the cell surface. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-02-10 |