File:Virion-Assembly-Factories-in-the-Nucleus-of-Polyomavirus-Infected-Cells-ppat.1002630.s003.ogv

English: Tomogram and model of a virus factory. A small virus array with tubular structures adjacent to the virions. A movie was compiled from a tilt-series (±60°) of a 300 nm thick section of PyV-infected 3T3 cells. The movie proceeds through the full tilt-series followed by 3-D modeling of the virus factory. Purple = full virions, red = empty virions and empty tubes, yellow = electron-dense, or “full,” tubes. In this array both full (dark “circles”/purple spheres) and empty capsids (empty “circles”/red spheres) are seen as the movie proceeds through the tilt series. When following the tubular structures (red or yellow cylinders) through the section, “bubble-like” protrusions appear along the electron-dense, or “full,” tubes. This tomogram also reveals the close association of tubular structures with virions. Single images are shown in Figure 2B (tomogram) and 2C (3-D model). (MP4)