File:Vinculin-controls-talin-engagement-with-the-actomyosin-machinery-ncomms10038-s4.ogv
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Vinculin-controls-talin-engagement-with-the-actomyosin-machinery-ncomms10038-s4.ogv (Ogg Theora video file, length 7.2 s, 540 × 360 pixels, 433 kbps, file size: 380 KB)
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[edit]DescriptionVinculin-controls-talin-engagement-with-the-actomyosin-machinery-ncomms10038-s4.ogv |
English: Supplementary Movie 3 Related to Figure 2. Recording of FLAP in FAs from NIH3T3 cells expressing the indicated PAGFP-talin construct +/- constitutively active vinculin. The top panel shows FLAP in control NIH3T3 cells. The fluorescence intensity plot shows the FLAP over time. Note the increased turnover of talΔR4-R10 and talΔR1-R10 compared to talinFL. The bottom panel shows FLAP in NIH3T3 cells co-expressing vinT12. Note the reduced turnover of talinFL and talΔR4-R10, but not talΔR1-R10, in the presence of constitutively active vinculin. Images were taken every 10 seconds for 5 minutes following photoactivation. Images are played back at 5 frames/s. Scale bar represents 5 μm. |
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Source | Video file from Atherton P, Stutchbury B, Wang D, Jethwa D, Tsang R, Meiler-Rodriguez E, Wang P, Bate N, Zent R, Barsukov I, Goult B, Critchley D, Ballestrem C (2015). "Vinculin controls talin engagement with the actomyosin machinery". Nature Communications. DOI:10.1038/ncomms10038. PMID 26634421. PMC: 4686655. | ||
Author | Atherton P, Stutchbury B, Wang D, Jethwa D, Tsang R, Meiler-Rodriguez E, Wang P, Bate N, Zent R, Barsukov I, Goult B, Critchley D, Ballestrem C | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:39, 30 October 2016 | 7.2 s, 540 × 360 (380 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 3 |
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Author | Atherton P, Stutchbury B, Wang D, Jethwa D, Tsang R, Meiler-Rodriguez E, Wang P, Bate N, Zent R, Barsukov I, Goult B, Critchley D, Ballestrem C |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Related to Figure 2. Recording of FLAP in FAs from NIH3T3 cells expressing the indicated PAGFP-talin construct +/- constitutively active vinculin. The top panel shows FLAP in control NIH3T3 cells. The fluorescence intensity plot shows the FLAP over time. Note the increased turnover of talΔR4-R10 and talΔR1-R10 compared to talinFL. The bottom panel shows FLAP in NIH3T3 cells co-expressing vinT12. Note the reduced turnover of talinFL and talΔR4-R10, but not talΔR1-R10, in the presence of constitutively active vinculin. Images were taken every 10 seconds for 5 minutes following photoactivation. Images are played back at 5 frames/s. Scale bar represents 5 μm. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-12-04 |