File:Video 1 Quail Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration.ogv
Video_1_Quail_Primordial_Germ_Cells_Contribute_to_and_Interact_With_the_Extracellular_Matrix_During_Early_Migration.ogv (Ogg Theora video file, length 9.3 s, 512 × 512 pixels, 1.08 Mbps, file size: 1.2 MB)
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Summary
[edit]| Description | Video S1. Live confocal microscope imaging of the germinal crescent region of an HH5 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). The antibody was directly conjugated to AlexaFluor 594, red). The 20x images, with 2x optical zoom, are maximum intensity projections of 3 optical slices (2.3 μm each) taken from the dorsal aspect at 1.5 min intervals. Total elapsed time is 26 min. Cell nuclei = H2B-Cerulean (blue), cell bodies = Dendra2 (green). While the cells are ubiquitously labeled by the transgene, those with low proliferation rates, such as PGCs, are brighter than the surrounding tissue. Anterior to top. Embryonic tissue to the lower right. Extra-embryonic tissue to the upper left. Scale bar = 25 μm. |
| Date | |
| Source | https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full#supplementary-material https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00035/full Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front. Cell Dev. Biol. 7:35. doi: 10.3389/fcell.2019.00035 |
| Author | Huss DJ, Saias S, Hamamah S, Singh JM, Wang J, Dave M, Kim J, Eberwine J and Lansford R |
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© 2019 Huss, Saias, Hamamah, Singh, Wang, Dave, Kim, Eberwine and Lansford. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:27, 11 July 2024 | 9.3 s, 512 × 512 (1.2 MB) | Rasbak (talk | contribs) | {{Information |description=Video S1. Live confocal microscope imaging of the germinal crescent region of an HH5 [Tg(hUbC:H2B-Cerulean-2A-Dendra2)] quail embryo that was injected with an antibody against fibronectin (abB3/D6). The antibody was directly conjugated to AlexaFluor 594, red). The 20x images, with 2x optical zoom, are maximum intensity projections of 3 optical slices (2.3 μm each) taken from the dorsal aspect at 1.5 min intervals. Total elapsed time is 26 min. Cell nuclei = H2B-Ceru... |
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