File:Usage-of-a-Localised-Microflow-Device-to-Show-that-Mitochondrial-Networks-Are-Not-Extensive-in-pone.0108601.s002.ogv

English: Multiple local application of FCCP depolarises only local but not distant mitochondria. FCCP (100 µM) was applied to two sites on the side of the same FDB fibre with the microflow pipette. An enlarged view of the fibre occupies the main area of the box and the whole fibre is shown enclosed in the red box on the right. Two timers are used. The first timer (white numbers) refers to the time (in minutes and seconds) at which FCCP application at the first site started while the second (blue numbers) refers to the time (in minutes and seconds) at which FCCP was applied to the second site. The vertical bar is a LUT where black indicates low TMRE signal and white represents high TMRE. The bright white halo shows the delivery of a sulforhodamine B-spiked Tyrode and indicates the area of the fibre exposed to the flow from the multifunctional pipette.