File:Usage-of-a-Localised-Microflow-Device-to-Show-that-Mitochondrial-Networks-Are-Not-Extensive-in-pone.0108601.s001.ogv

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English: Local application of FCCP depolarises local but not distant mitochondria. FCCP (100 µM) was applied with the microflow pipette to the end of a FDB fibre. An enlarged view of the fibre occupies the main area of the box and the whole fibre is shown enclosed in the red box at the lower right. Time refers to the time (in minutes and seconds) at which FCCP application started. The vertical bar is a LUT where black indicates low TMRE signal and white represents high TMRE. The bright white halo at times −00∶21 and −00∶14 is the delivery of a sulforhodamine B-spiked Tyrode solution indicating the area of the fibre exposed to the flow from the multifunctional pipette. White horizontal calibration bar represents 50 µm.
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Source Movie S1 from Bruton J, Jeffries G, Westerblad H (2014). "Usage of a Localised Microflow Device to Show that Mitochondrial Networks Are Not Extensive in Skeletal Muscle Fibres". PLOS ONE. DOI:10.1371/journal.pone.0108601. PMID 25259575. PMC: 4178183.
Author Bruton J, Jeffries G, Westerblad H
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Date/TimeThumbnailDimensionsUserComment
current15:23, 3 October 20144.4 s, 1,400 × 1,330 (3.76 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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