File:Tunneling-Nanotubes-Provide-a-Unique-Conduit-for-Intercellular-Transfer-of-Cellular-Contents-in-pone.0033093.s006.ogv

English: (Time-lapse). Migrating mesothelioma cells demonstrate significant formation of TnTs. A scratch assay was performed by growing MSTO-211H cells for 72 hours in low-serum, hyperglycemic, acidic RPMI-1640. A micropipette tip was used to create a gap, and the medium was replaced with fresh medium. Time-lapse brightfield confocal microscopy was performed every 30 minutes for 24 hours, demonstrating formation of multiple TnTs along the advancing cell front. White arrows were inserted and appear at 11.5 hours and 14 hours to highlight TnTs. The white arrowhead appearing at 16 hours tracks apparent movement of cargo in a TnT.