File:Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s012.ogv
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Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s012.ogv (Ogg Theora video file, length 26 s, 306 × 150 pixels, 132 kbps, file size: 418 KB)
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[edit]DescriptionTransportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s012.ogv |
English: HMM-propelled actin filaments with streptavidin coated quantum dots attached to the filaments via biotinylated actin. The filaments are dually labelled and observed using a filter that simultaneously allows observation of Alexa-488 and quantum dots (emission maximum at 605 nm). Note, large number of quantum dots (>5) attached to, presumably, two cross-linked filaments at top left (no free biotin in assay solution). Note further that one filament with two quantum dots (one at very front) that moves to the right, suddenly stops and begins to rotate counter-clockwise for five consecutive turns. This indicates unconstrained rotation around one of the quantum dots as an axis and continuous driving of this rotation by appropriately located HMM molecules. From series of experiments in Fig.3 (a80 solution). Frame rate: 5 s−1 (real time). Image size: 46×33 µm2. |
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Source | Movie S4 from Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A (2013). "Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments". PLOS ONE. DOI:10.1371/journal.pone.0055931. PMID 23437074. | ||
Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:41, 4 March 2013 | 26 s, 306 × 150 (418 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |