File:Transcriptional-Activation-of-the-Adenoviral-Genome-Is-Mediated-byCapsid-Protein-VI-ppat.1002549.s009.ogv
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Transcriptional-Activation-of-the-Adenoviral-Genome-Is-Mediated-byCapsid-Protein-VI-ppat.1002549.s009.ogv (Ogg Theora video file, length 5.0 s, 612 × 204 pixels, 1.28 Mbps, file size: 779 KB)
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[edit]DescriptionTranscriptional-Activation-of-the-Adenoviral-Genome-Is-Mediated-byCapsid-Protein-VI-ppat.1002549.s009.ogv |
English: Microinjection of recombinant protein VI-wt displaces Daxx from PML bodies to the cytoplasm. U2OS cells were cotransfected with Daxx-mCherry (left panel) and PML-GFP (middle panel) expression plasmids (superimposed signal on the right) and cultivated at 37°C on a heated stage in CO2 stabilized medium attached to a SP5 confocal microscope equipped with a microinjection device (Eppendorf). The left cell of two cotransfected cells with equal expression levels of both proteins was then microinjected into the cytoplasm using recombinant protein VI at a final concentration of 0.3 µg/µl. Prior to injection, cells were imaged using the SP5 confocal microscope at 20 s intervals for 10 frames at a single optical section with pinhole setting of two using a 20× magnification and maximum resolution. Injection was performed manually under optical control in DIC mode and immediately after images were taken at 20 s intervals for further 40 frames without changing the optical setting to minimize the time between injection and imaging (time delay ∼1–2 min). Note the cytoplasmic accumulation of the Daxx signal following injection. |
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Source | Video S1 from Schreiner S, Martinez R, Groitl P, Rayne F, Vaillant R, Wimmer P, Bossis G, Sternsdorf T, Marcinowski L, Ruzsics Z, Dobner T, Wodrich H (2012). "Transcriptional Activation of the Adenoviral Genome Is Mediated by Capsid Protein VI". PLOS Pathogens. DOI:10.1371/journal.ppat.1002549. PMID 22427750. PMC: 3303589. | ||
Author | Schreiner S, Martinez R, Groitl P, Rayne F, Vaillant R, Wimmer P, Bossis G, Sternsdorf T, Marcinowski L, Ruzsics Z, Dobner T, Wodrich H | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:22, 27 June 2013 | 5.0 s, 612 × 204 (779 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Schreiner S, Martinez R, Groitl P, Rayne F, Vaillant R, Wimmer P, Bossis G, Sternsdorf T, Marcinowski L, Ruzsics Z, Dobner T, Wodrich H |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-02 |