File:Transcriptional, spatiotemporal and paracrine signatures of human pregranulosa cells.jpg
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| Description | Figure 4. Transcriptional, spatiotemporal and paracrine signatures of human pregranulosa cells. a, Dot plots show the variance-scaled, log-transformed expression of genes (x-axis) characteristic of ovarian supporting cells (y-axis) in human scRNA-seq data. Top layer groups marker genes by categories. b, Spatial mapping of granulosa cell types from the scRNA-seq human dataset to spatial transcriptomics slide of 11, 14, 17 and 19 PCW ovaries using cell2location; n = 2. Estimated cell abundance (colour intensity) for OSE, preGC-I, preGC-IIa, preGC-IIb and developing granulosa cells (colour) in each Visium spot shown over the haematoxylin and eosin (H&E) images. The black rectangles highlight enlarged ovarian regions with forming follicles (top right). Schematic representation of the spatial organization of pregranulosa cell states in the human ovary (bottom right). Scale bars 1 mm (left) and 50 µm in magnified regions (right). c, Heatmaps showing expression of selected TFs across human, macaque and mouse ovarian supporting cells. Colour proportional to scaled log-transformed expression. For human ovarian supporting cells only, 'o' denotes TF whose binding motifs are differentially accessible (that is, TF can bind their potential targets); 'a' denotes TF whose targets are also differentially expressed (that is, differentially activated TF) and asterisk denotes TF that meets both 'o' and 'a' conditions. Conservation heatmap (right) highlights significant overexpression (log2 fold change > 0 and FDR < 0.05) in each species. TFs whose upregulation is conserved across species are highlighted with bold/coloured labels. d, Dot plots showing scaled z scored expression of genes coding for interacting ligand–receptor proteins (CellPhoneDB) in supporting and germ cell states in the outer cortex, inner cortex and primordial follicles. Specific interacting partners are linked with a matching symbol. CoelEpi, coelomic epithelium; Expr, expressed; FGC, fetal germ cells; preGC, pregranulosa cells; granulosa, developing granulosa. |
| Date | |
| Source | https://www.nature.com/articles/s41586-022-04918-4/ Single-cell roadmap of human gonadal development. Nature 607, 540–547 (2022). https://doi.org/10.1038/s41586-022-04918-4 |
| Author | Garcia-Alonso, L., Lorenzi, V., Mazzeo, C.I. et al. |
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| current | 17:17, 10 July 2024 | | 2,118 × 1,595 (1.91 MB) | Rasbak (talk | contribs) | {{Information |description=Figure 4. Transcriptional, spatiotemporal and paracrine signatures of human pregranulosa cells. a, Dot plots show the variance-scaled, log-transformed expression of genes (x-axis) characteristic of ovarian supporting cells (y-axis) in human scRNA-seq data. Top layer groups marker genes by categories. b, Spatial mapping of granulosa cell types from the scRNA-seq human dataset to spatial transcriptomics slide of 11, 14, 17 and 19 PCW ovaries using cell2location; n = 2... |
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