File:Three-dimensional-structural-dynamics-and-fluctuations-of-DNA-nanogold-conjugates-byindividual-ncomms11083-s2.ogv
Three-dimensional-structural-dynamics-and-fluctuations-of-DNA-nanogold-conjugates-byindividual-ncomms11083-s2.ogv (Ogg Theora video file, length 3 min 20 s, 384 × 384 pixels, 831 kbps, file size: 19.77 MB)
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English: Supplementary Movie 1 Individual particle electron tomography (IPET) 3D reconstruction of an
individual DNA-nanogold conjugate. A tilt series of the electron microscopic survey views of an individual molecule containing two 5.5-nm nanogold particles conjugated with 84-base-pair dsDNA, was prepared via optimized negative staining (OpNS). The higher-magnification views of an individual DNA-nanogold conjugate particle show its detailed structure. Via the IPET 3D reconstruction, the tilt images of the targeted conjugates were aligned to a global center and reconstructed onto a 3D density map. By overlaying the 3D density map (in gray) with its inverted map (in gold), the resulting map showed the structure of the DNA nanogold conjugate. By flexibly docking a standard dsDNA model into the fabric density portion via targeted molecular dynamics (TMD) simulation, a new conformation of dsDNA and nanogold was achieved. We revealed the flexibility of the DNA-nanogold conjugates by aligning a total of 14 structures of the DNA-nanogold conjugates. |
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| Source | Video file from Zhang L, Lei D, Smith J, Zhang M, Tong H, Zhang X, Lu Z, Liu J, Alivisatos A, Ren G (2016). "Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography". Nature Communications. DOI:10.1038/ncomms11083. PMID 27025159. PMC: 4820932. | ||
| Author | Zhang L, Lei D, Smith J, Zhang M, Tong H, Zhang X, Lu Z, Liu J, Alivisatos A, Ren G | ||
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 4.0 International license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:35, 28 October 2016 | 3 min 20 s, 384 × 384 (19.77 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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