File:Three-color-single-molecule-imaging-shows-WASP-detachment-from-Arp23-complex-triggers-actin-elife01008v001.ogv
Three-color-single-molecule-imaging-shows-WASP-detachment-from-Arp23-complex-triggers-actin-elife01008v001.ogv (Ogg Theora video file, length 1 min 50 s, 200 × 200 pixels, 144 kbps, file size: 1.89 MB)
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[edit]DescriptionThree-color-single-molecule-imaging-shows-WASP-detachment-from-Arp23-complex-triggers-actin-elife01008v001.ogv |
English: The diVCA-stimulated actin branch formation event shown in Figure 2B–C . Red: Arp2/3-SNAP649 (5 nM in solution); green: Cy3-diVCA (5 nM); blue: actin-AF488 (1 µM, 10% labeled). Arp2/3 complex and diVCA images were recorded every 0.05 s; actin images were recorded every ∼12 s. Playback rate: real time. Bar: 1 μm. Many diVCA-Arp2/3 complexes are observed to transiently associate with actin filaments. One such nascent branch complex (yellow arrowhead) releases diVCA shortly after it appears, leaving Arp2/3 complex stably associated with the mother filament (red arrowhead), where it subsequently initiates daughter filament elongation.
DOI: https://dx.doi.org/10.7554/eLife.01008.009 |
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Source | Video 1. from Smith B, Padrick S, Doolittle L, Daugherty-Clarke K, Correa I, Xu M, Goode B, Rosen M, Gelles J (2013). "Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation". eLife. DOI:10.7554/eLife.01008. PMID 24015360. PMC: 3762362. | ||
Author | Smith B, Padrick S, Doolittle L, Daugherty-Clarke K, Correa I, Xu M, Goode B, Rosen M, Gelles J | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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current | 11:02, 15 September 2013 | 1 min 50 s, 200 × 200 (1.89 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | The diVCA-stimulated actin branch formation event shown in Figure 2B?C . |
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Author | Smith B, Padrick S, Doolittle L, Daugherty-Clarke K, Correa I, Xu M, Goode B, Rosen M, Gelles J |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09-03 |