File:Three-color-single-molecule-imaging-shows-WASP-detachment-from-Arp23-complex-triggers-actin-elife01008v001.ogv

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Three-color-single-molecule-imaging-shows-WASP-detachment-from-Arp23-complex-triggers-actin-elife01008v001.ogv ‎(Ogg Theora video file, length 1 min 50 s, 200 × 200 pixels, 144 kbps, file size: 1.89 MB)

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English: The diVCA-stimulated actin branch formation event shown in Figure 2B–C . Red: Arp2/3-SNAP649 (5 nM in solution); green: Cy3-diVCA (5 nM); blue: actin-AF488 (1 µM, 10% labeled). Arp2/3 complex and diVCA images were recorded every 0.05 s; actin images were recorded every ∼12 s. Playback rate: real time. Bar: 1 μm. Many diVCA-Arp2/3 complexes are observed to transiently associate with actin filaments. One such nascent branch complex (yellow arrowhead) releases diVCA shortly after it appears, leaving Arp2/3 complex stably associated with the mother filament (red arrowhead), where it subsequently initiates daughter filament elongation.

DOI:

https://dx.doi.org/10.7554/eLife.01008.009
Date
Source Video 1. from Smith B, Padrick S, Doolittle L, Daugherty-Clarke K, Correa I, Xu M, Goode B, Rosen M, Gelles J (2013). "Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation". eLife. DOI:10.7554/eLife.01008. PMID 24015360. PMC: 3762362.
Author Smith B, Padrick S, Doolittle L, Daugherty-Clarke K, Correa I, Xu M, Goode B, Rosen M, Gelles J
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Date/TimeThumbnailDimensionsUserComment
current11:02, 15 September 20131 min 50 s, 200 × 200 (1.89 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 53 kbps Completed 08:14, 17 July 2024 21 s
Streaming 240p (VP9) 57 kbps Completed 19:49, 18 December 2023 13 s
WebM 360P 64 kbps Completed 17:26, 2 December 2023 4.0 s
Streaming 144p (MJPEG) 421 kbps Completed 03:23, 10 November 2023 2.0 s

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