File:The-structure-of-mollusc-larval-shells-formed-in-the-presence-of-the-chitin-synthase-inhibitor-1472-6807-7-71-S15.ogv

English: Additional File 15 Video microscopy of 15-day larvae after 7 days of NikkomycinZ treatment. Video microscopic (32× objective) demonstration of the behaviour and phenotype of 15-day larvae treated from the 8th day on with the chitin synthase inhibitor NikkomycinZ. Even in later developmental stages, NikkomycinZ induced characteristic effects on the shell development of living individuals. Note that the previously straight hinge (see additional files 7 &8) appears now curved. This indicates that NikkomycinZ interferes with the remodelling of the hinge region. The fact that shells are much smaller than in the untreated control cultures (additional file 8) suggests that either the solubility of the newly formed shell is increased, or lateral shell growth is blocked by the chitin synthase inhibitor drug. Note that also shell remnants of larvae that died at undefined age are present in this data set.