File:The-mitotic-kinesin-14-KlpA-contains-a-context-dependent-directionality-switch-ncomms13999-s5.ogv
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The-mitotic-kinesin-14-KlpA-contains-a-context-dependent-directionality-switch-ncomms13999-s5.ogv (Ogg Theora video file, length 20 s, 199 × 332 pixels, 3.29 Mbps, file size: 7.82 MB)
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[edit]DescriptionThe-mitotic-kinesin-14-KlpA-contains-a-context-dependent-directionality-switch-ncomms13999-s5.ogv |
English: Supplementary Movie 4 KlpA moves processively toward the plus end on single microtubules. Movie showing that individual KlpA molecules (green) move processively toward the plus ends on surface-immobilized microtubules (red). Microtubules are fluorescently labeled with Hilyte 647 and polarity-marked with a bright plus end. Top: the microtubule channel; Middle: the GFP-KlpA channel; Bottom: the overlay of the microtubule and GFP-KlpA channel. The movie corresponds to Fig. 2c. Arrowhead indicates the plus end of the polarity microtubule used for generating the leftmost kymograph in Fig. 2c. |
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Source | Video file from Popchock A, Tseng K, Wang P, Karplus P, Xiang X, Qiu W (2017). "The mitotic kinesin-14 KlpA contains a context-dependent directionality switch". Nature Communications. DOI:10.1038/ncomms13999. PMID 28051135. PMC: 5216134. | ||
Author | Popchock A, Tseng K, Wang P, Karplus P, Xiang X, Qiu W | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:23, 31 January 2017 | 20 s, 199 × 332 (7.82 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 4 |
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Author | Popchock A, Tseng K, Wang P, Karplus P, Xiang X, Qiu W |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | KlpA moves processively toward the plus end on single microtubules. Movie showing that individual KlpA molecules (green) move processively toward the plus ends on surface-immobilized microtubules (red). Microtubules are fluorescently labeled with Hilyte 647 and polarity-marked with a bright plus end. Top: the microtubule channel; Middle: the GFP-KlpA channel; Bottom: the overlay of the microtubule and GFP-KlpA channel. The movie corresponds to Fig. 2c. Arrowhead indicates the plus end of the polarity microtubule used for generating the leftmost kymograph in Fig. 2c. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2017-01-04 |