File:The-mitotic-kinesin-14-KlpA-contains-a-context-dependent-directionality-switch-ncomms13999-s10.ogv
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The-mitotic-kinesin-14-KlpA-contains-a-context-dependent-directionality-switch-ncomms13999-s10.ogv (Ogg Theora video file, length 1.9 s, 201 × 248 pixels, 505 kbps, file size: 119 KB)
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[edit]DescriptionThe-mitotic-kinesin-14-KlpA-contains-a-context-dependent-directionality-switch-ncomms13999-s10.ogv |
English: Supplementary Movie 9 KlpA exhibits opposite directional preference inside and outside the antiparallel microtubule overlap. Movies showing that GFP-KlpA molecules (green) move preferentially toward the plus end of the track microtubule (blue) outside the antiparallel overlap and collectively transport the cargo microtubule (red) toward the minus end of the track microtubule. The cargo and track microtubule were both polarity-marked with a bright plus end, and fluorescently labeled with TMR and Hilyte 647 respectively. From Top to Bottom: the track microtubule channel (the movie was made from a single snapshot of the track microtubule); the GFP-KlpA channel; the cargo microtubule channel; and the overlay of the GFP-KlpA and cargo microtubule channels. Movie corresponds to Fig. 4b. Arrowhead indicates the plus end of the track microtubule used for generating the kymograph in Fig. 4b. |
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Source | Video file from Popchock A, Tseng K, Wang P, Karplus P, Xiang X, Qiu W (2017). "The mitotic kinesin-14 KlpA contains a context-dependent directionality switch". Nature Communications. DOI:10.1038/ncomms13999. PMID 28051135. PMC: 5216134. | ||
Author | Popchock A, Tseng K, Wang P, Karplus P, Xiang X, Qiu W | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 21:24, 31 January 2017 | 1.9 s, 201 × 248 (119 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 9 |
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Author | Popchock A, Tseng K, Wang P, Karplus P, Xiang X, Qiu W |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | KlpA exhibits opposite directional preference inside and outside the antiparallel microtubule overlap. Movies showing that GFP-KlpA molecules (green) move preferentially toward the plus end of the track microtubule (blue) outside the antiparallel overlap and collectively transport the cargo microtubule (red) toward the minus end of the track microtubule. The cargo and track microtubule were both polarity-marked with a bright plus end, and fluorescently labeled with TMR and Hilyte 647 respectively. From Top to Bottom: the track microtubule channel (the movie was made from a single snapshot of the track microtubule); the GFP-KlpA channel; the cargo microtubule channel; and the overlay of the GFP-KlpA and cargo microtubule channels. Movie corresponds to Fig. 4b. Arrowhead indicates the plus end of the track microtubule used for generating the kymograph in Fig. 4b. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2017-01-04 |