File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s009.ogv
The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s009.ogv (Ogg Theora video file, length 44 s, 560 × 560 pixels, 653 kbps, file size: 3.43 MB)
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[edit]DescriptionThe-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s009.ogv |
English: (Related to Fig. S3A). M90T Shigella-dependent macrophage death. A 72 hpf mpeg1:G/U:mCherry larva was injected iv with a sublethal dose of GFP-Shigella (about 3000 to 4000 bacteria) and live imaged every 1′55″ from 20 mpi (t = 0 on the movie) until 6h20pi (t = 6h59hpi on the movie) by confocal fluorescence microscopy. 5 mCherry+ macrophages harboring GFP-Shigella were tracked over time (red phagocyte with green bacteria, white arrows and labeled with number 1 to 5) progressively round up and undergoes cell death (as suggested by the loss of the red label) presumably killed by the GFP-Shigella (follow single macrophage tracking on the movie). Note that dying macrophages seem to burst, releasing in the extracellular milieu live GFP-Shigella. Note also that, at the end on the acquisition, there are still GFP-Shigella in the blood and adjacent mesenchyme. The maximum intensity projection from 26 planes every 2 µm is shown. Scale bar, 20 µm. |
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Source | Video S3 from Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E (2013). "The Zebrafish as a New Model for the In Vivo Study of Shigella flexneri Interaction with Phagocytes and Bacterial Autophagy". PLOS Pathogens. DOI:10.1371/journal.ppat.1003588. PMC: 3764221. | ||
Author | Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E | ||
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current | 20:35, 14 September 2013 | 44 s, 560 × 560 (3.43 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | (Related to Fig. S3A). M90T Shigella-dependent macrophage death. A 72 hpf mpeg1:G/U:mCherry larva was injected iv with a sublethal dose of GFP-Shigella (about 3000 to 4000 bacteria) and live imaged every 1?55? from 20 mpi (t? |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09 |