File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s008.ogv

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The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s008.ogv (Ogg Theora video file, length 1 min 0 s, 560 × 560 pixels, 665 kbps, file size: 4.77 MB)

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English: (Related to Fig. S3A). T3SS- Shigella are rapidly degraded by macrophages. A 72 hpf Tg(mpeg1:Gal4FFgl25/UAS:kaede) larva was injected iv with T3SS- DsRed-Shigella (about 6000 to 8000 bacteria) and live imaged every 1′23″ from 20 mpi (t = 0 on the movie) until 6h20pi (t = 6h58hpi on the movie) by confocal fluorescence microscopy. 6 GFP+ macrophages harboring T3SS- DsRed-Shigella were tracked over time (green phagocyte with red bacteria, white arrows and labeled 1 to 6) progressively kill and degrade the engulfed bacteria (as indicated by the diffuse red label accumulating in their phagosomes) (follow single macrophage tracking on the movie). Note that T3SS- Shigella do not cause macrophage death nor these bacteria seem to invade the cytosol of macrophages. Note also that, at the end on the acquisition, all the T3SS- Shigella were cleared from the blood. The maximum intensity projection from 26 planes every 2 µm is shown. Scale bar, 20 µm.
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Source Video S2 from Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E (2013). "The Zebrafish as a New Model for the In Vivo Study of Shigella flexneri Interaction with Phagocytes and Bacterial Autophagy". PLOS Pathogens. DOI:10.1371/journal.ppat.1003588. PMC: 3764221.
Author Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E
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current20:34, 14 September 20131 min 0 s, 560 × 560 (4.77 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 250 kbps Completed 23:07, 21 October 2018 20 s
VP9 360P 145 kbps Completed 23:07, 21 October 2018 24 s
VP9 240P 82 kbps Completed 23:07, 21 October 2018 15 s
WebM 360P 507 kbps Completed 20:35, 14 September 2013 24 s
QuickTime 144p (MJPEG) Not ready Unknown status

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