File:The-Use-of-Spinning-Disk-Confocal-Microscopy-for-the-Intravital-Analysis-of-Platelet-Dynamics-in-pone.0025109.s016.ogv
The-Use-of-Spinning-Disk-Confocal-Microscopy-for-the-Intravital-Analysis-of-Platelet-Dynamics-in-pone.0025109.s016.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 17 s, 512 × 512 pixels, 391 kbps overall, file size: 812 KB)
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[edit]DescriptionThe-Use-of-Spinning-Disk-Confocal-Microscopy-for-the-Intravital-Analysis-of-Platelet-Dynamics-in-pone.0025109.s016.ogv |
English: Circulating platelet-neutrophil aggregates within the liver of an LPS treated mouse. Intravital visualization of a liver from a C57BL/6 mouse 4 h after LPS treatment (1 mg/kg i.v.). Two neutrophils coated in adherent platelets can be seen circulating through the liver microvasculature (neutrophils enter FOV at t = 4 s and t = 11 s) (note: the platelet aggregates appear ahead of the neutrophil due to the sequential capture of individual fluorescent channels). Platelets labelled with PE-conjugated anti-CD49b (red); neutrophils labelled with Alexa Fluor 647-conjugated anti-Gr-1 (blue); green is liver autofluorescence to illustrate vessels. Objective: UPLANSAPO 20×/0.70. Scale bar, 20 µm. |
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Source | Movie S8 from Jenne C, Wong C, Petri B, Kubes P (2011). "The Use of Spinning-Disk Confocal Microscopy for the Intravital Analysis of Platelet Dynamics in Response to Systemic and Local Inflammation". PLOS ONE. DOI:10.1371/journal.pone.0025109. PMID 21949865. PMC: 3176312. | ||
Author | Jenne C, Wong C, Petri B, Kubes P | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 07:18, 21 October 2012 | 17 s, 512 × 512 (812 KB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Author | Jenne C, Wong C, Petri B, Kubes P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Circulating platelet-neutrophil aggregates within the liver of an LPS treated mouse. Intravital visualization of a liver from a C57BL/6 mouse 4 h after LPS treatment (1 mg/kg i.v.). Two neutrophils coated in adherent platelets can be seen circulating through the liver microvasculature (neutrophils enter FOV at t? |
Software used | |
Date and time of digitizing | 2011 |
Language | English |
- Monoclonal antibodies
- Videos of platelets
- Videos of brain
- Cell adhesion
- Vascular endothelium
- Flow cytometry
- Videos of inflammations
- Integrin alpha2
- Lipopolysaccharides
- Videos of livers
- Inbred C57BL mice
- Transgenic mice
- Confocal microscopic movies from Open Access Journals
- Videos of fluorescence microscopy
- Videos of muscles
- Platelet adhesiveness
- Platelet membrane glycoprotein iib
- Media from PLOS ONE