File:The-RNA-Binding-Protein-Igf2bp1-Is-Required-for-Zebrafish-RGC-Axon-Outgrowth-In-Vivo-pone.0134751.s007.ogv
The-RNA-Binding-Protein-Igf2bp1-Is-Required-for-Zebrafish-RGC-Axon-Outgrowth-In-Vivo-pone.0134751.s007.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 25 s, 320 × 318 pixels, 576 kbps overall, file size: 1.73 MB)
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[edit]DescriptionThe-RNA-Binding-Protein-Igf2bp1-Is-Required-for-Zebrafish-RGC-Axon-Outgrowth-In-Vivo-pone.0134751.s007.ogv |
English: Editing used to isolate retina for counting AO-positive cells. 3D rendering, generated in FluoRender, of confocal z-stack (40x water immersion lens) of acridine orange staining, rotating around Y-axis, in a live 31 hpf uninjected embryo (similar to Fig 4I). The extraocular tissues and the lens (red) were erased with the paintbrush tool in ImageJ. The resulting stack (green) represented the retina alone. AO positive cells in each retina were counted in 3D using Imaris. |
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Source | S3 Movie from Gaynes J, Otsuna H, Campbell D, Manfredi J, Levine E, Chien C (2015). "The RNA Binding Protein Igf2bp1 Is Required for Zebrafish RGC Axon Outgrowth In Vivo". PLOS ONE. DOI:10.1371/journal.pone.0134751. PMID 26325373. PMC: 4556669. | ||
Author | Gaynes J, Otsuna H, Campbell D, Manfredi J, Levine E, Chien C | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 23:19, 15 September 2015 | 25 s, 320 × 318 (1.73 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Editing used to isolate retina for counting AO-positive cells. |
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Author | Gaynes J, Otsuna H, Campbell D, Manfredi J, Levine E, Chien C |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | 3D rendering, generated in FluoRender, of confocal z-stack (40x water immersion lens) of acridine orange staining, rotating around Y-axis, in a live 31 hpf uninjected embryo (similar to Fig 4I). The extraocular tissues and the lens (red) were erased with the paintbrush tool in ImageJ. The resulting stack (green) represented the retina alone. AO positive cells in each retina were counted in 3D using Imaris. |
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Language | English |